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. 2016 Nov 22;13(1):45–50. doi: 10.3892/ol.2016.5410

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Copyright: © Tajima et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — (A) Structural analysis of the sugar chains of LacCer, as indicated by arrows, by TLC following enzyme treatment with α-galactosidase. Upon treatment with the enzyme, both T1 and T2 changed to LacCer, and both were observed to be Gb₃Cer with a terminal α-galactose. (B) Analysis of the composition of the long-chain base by TLC. Dihydrosphingosine was noticed to be the principal component in both T1 and T2, while sphingosine or phytosphingosine were not detected in either of them. However, TLC analysis revealed that the ceramide of G1 was composed of sphingosine, dihydrosphingosine and phytosphingosine, while G2 contained just sphingosine and dihydrosphingosine. TLC, thin-layer chromatography; LacCer, lactosylceramide; Gb₃Cer, globotriaosylceramide.