Figure 3.

Changes in expression levels of genes involved in metabolism and cyclin D1. HLF cells were cultured in HSM and RNA was isolated. Reverse transcription-quantitative polymerase chain reaction was performed. Graphs show the REL, normalized against human adult liver cells, of (A) galactokinase 1, (B) galactokinase 2, (C) ornithine carbamoyltransferase, (D) phosphoenolpyruvate carboxykinase, (E) glucose-6-phosphatase and (F) cyclin D1; *P<0.05 as compared with adult, **P<0.05 as compared with D0 (n=3). D0, day 0 after culture in HSM; D3, three days after culture in HSM; D7, seven days after culture in HSM; adult, human adult liver; HSM, hepatocyte selection medium; REL, relative expression levels.