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(A) Co-culture of HLF with HUVECs was established as an in vitro model of hepatoma tissues. Cells were observed under the microscope at (B) 18 h and (C) 60 h after the medium was changed to hepatocyte selection medium. Two cells started to die at 18 h. Almost all the cells died at 60 h. Original magnification, ×100; scale bar, 50 µm; arrow, HUVECs; arrowhead, HLF. HUVECs, human umbilical vascular endothelial cells.
