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. 2016 Nov 23;13(1):329–338. doi: 10.3892/ol.2016.5423

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — miR-101 decreases A549 cell proliferation and induces S-phase arrest in vitro. (A) The effects of miR-101 on A549 cell proliferation were determined by MTT assay at 24, 48 and 72 h. (B) Representative results of colony formation of A549 cells following miR-101 overexpression. (C) The cell cycle was determined in A549 cells 48 h after transfection. (D) Analysis of the expression of S-phase regulatory protein in A549 cells following transfection with miR-ctrl and miR-101 overexpression construct. (E) MTT was used to assay the effects of miR-101 inhibitor on the proliferation of A549 cells. (F) The growth of A549 cells was detected by colony formation following transfection of inhibitor. (G) The cell cycle was determined by transfecting with inhibitor or its control. (H) Expression of cell cycle regulators 48 h after transfection with inhibitor and control (*P<0.05, **P<0.01, Student's t-test).