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. 2016 Nov 23;13(1):159–166. doi: 10.3892/ol.2016.5428

Figure 2.

Figure 2.

Expression of Treg cells and Th17 cells using immunofluorescence staining. (A) Treg cells or Th17 cells were stained by DAPI (Blue), CD4 (Green) and Foxp3 (Red; IL-17 for Th17 cells) in benign tumor tissues. (B) Treg cells or Th17 cells were stained by DAPI (Blue), CD4 (Green) and Foxp3 (Red; IL-17 for Th17 cells) in EOC tissues. The yellow arrows indicate TAMs. The frequency of (C) Treg cells, frequency of (D) Th17 cells and (E) ratio of Treg/Th17 cells were significantly higher in EOC tissues, compared with the benign tumor tissues. Scale bar=50 µm. EOC, epithelial ovarian cancer; TAM, tumor-associated macrophage; Treg, regulatory T cell; Th17, T helper cell 17; IL-17: interleukin 17.