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. 2016 Nov 11;13(1):119–128. doi: 10.3892/ol.2016.5381

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Copyright: © Sophonnithiprasert et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Increases of cell surface phosphatidyl-serine presentation in HeLa cells by goniothalamin. The Cell surface presentation of phosphatidyl-serine presentation on HeLa cells treated with 15 µM goniothalamin for 12 h was detected via Annexin V assessment. The percentage in each quadrant indicates the levels of normal cells (annexin V-FITC⁻/propidium iodide⁻), early apoptotic cells (annexin V-FITC⁺/propidium iodide⁻), late apoptotic cells (annexin V-FITC⁺/propidium iodide⁺) and necrotic cells (annexin V-FITC⁻/propidium iodide⁺). These results indicated that goniothalamin induced phosphatidyl-serine exposure, indicative of apoptosis induction. The results shown are representative data from three independent experiments. Percentages of apoptotic cells are presented as the mean ± standard deviation from at least three independent experiments. *P<0.05, vs. control. FITC, fluorescein isothiocyanate.