Figure 6.

MAPK activation is associated with the induction of apoptosis. HeLa cells were treated with 15 µM of goniothalamin for 3, 6, 9 and 12 h. Immunoblotting assays of (A) MAPK pathway mediators, (B) apoptosis mediators, and (C) p53, antiapoptotic Bcl2, proapoptotic Bax and Bad molecules and phosphorylated Bcl2 (Ser70). The results indicated that goniothalamin induced apoptosis in HeLa cells, associated with activation of the MAPK pathway. Images shown are representative from three independent experiments. MAPK, mitogen-activated protein kinase; SAPK, stress-activated protein kinase; JNK, c-Jun NH₂-terminal kinase; Erk1/2. extracellular signal-regulated kinase 1/2; PARP, poly ADP ribose polymerase; Bcl2, B cell lymphoma 2; Bax, Bcl2-associated X protein; Bad, Bcl2-associated death promoter.