Immunoblotting analysis of TCTP null mutant (TCTP−/−) embryos (E9.5) with anti-TCTP, Oct-4, Pax-2 and β-actin antibodies. Lane 1 and 2, control mouse embryos at E9.5; lane 3 and 4, TCTP null mutant (TCTP−/−) embryos at E9.5. TCTP, translationally-controlled tumor protein; Oct-4, octamer-binding transcription factor 4; E9.5, embryos at day 9.5.