Figure 5.

In vitro antibiotic efficacy experiments. (A) The indicated Mycobacterium tuberculosis H37Rv strains were grown in the presence of carbenicillin and clavulanate (left panel) or meropenem (right panel) at the indicated concentrations. Bacterial survival in the presence of antibiotic was calculated in comparison to untreated bacterial cells. Wild‐type PonA1, wt; TG‐mutant PonA1, TG‐; TP‐mutant PonA1, TP‐; double TG‐ and TP‐mutant PonA1, TG‐TP‐. Data were collected in duplicate for each strain and are representative of two experiments. Error bars represent SD. The asterisk indicates P‐value of < 0.05 and was determined by an unpaired, two‐tailed t‐test. (B) M. smegmatis mc²155 strains expressing wild‐type PonA1 (wt, nonoutlined squares) or transpeptidase mutant PonA1 (TP‐, outlined circles) were grown in the presence of penicillin V and clavulanate (left panel) or meropenem (right panel) at the indicated concentrations. Untreated control cells are shown in gray for both strains. Data were collected in triplicate (for carbenicillin data) or duplicate (for penicillin V data) for each strain and are representative of two experiments. Error bars represent SEM.