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Relative mRNA expression levels of VEGF in leukemia cells. VEGF overexpression and knockdown were achieved in the three cell lines, as confirmed by reverse transcription-polymerase chain reaction and western blotting. The background expression of VEGF was at a high level in leukemia cells and genetic manipulation resulted in significantly altered expression of VEGF in the cells (*P<0.01). VEGFa-O, overexpression of VEGF gene; VEGFa-shRNA, knockdown of VEGF; NC-LV, controls.
