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. 2016 Dec 5;13(1):187–193. doi: 10.3892/etm.2016.3954

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — LMP2A RNAi treatment induced GT38 cells into apoptosis which was detected by FCM using Annexin V-FITC/PI double staining. (A) Compared with the CON and NC groups, LMP2A RNAi significantly promoted cell apoptosis in the KD group (*P<0.05). Data are presented as the mean plus standard deviation (n=6). (B) FCM data show that the apoptotic rate of cells with downregulation of LMP2A is significantly increased. LMP2A, latent membrane protein 2A; RNAi, RNA interference; FCM, flow cytometry; FITC, fluorescein isothiocyanate; PI, propidium iodide; CON, blank control; NC, negative control; KD, LMP2A knockdown.