Figure 2.

Hoxd10 is a direct target of miR-376b. (A) Hoxd10 3′-UTR contains one predicted miR-376b binding site. The figure shows predicted duplex formations between Hoxd10 3′UTR and miR-376b. The sites of target mutagenesis are indicated in blue. (B) Quantitative polymerase chain reaction analysis of miR-376b in 293 cells after enforced expression of miR-376b in 293 cells. (C) Relative repression of luciferase expression was standardized to a transfection control (n=4; **P<0.01 vs. miR-NC control). (D) Hoxd10 expression in 4T1 cells that transfected with miR-NC or miR-376b-mimic was detected by western blot analysis. The density of Hoxd10/GAPDH was analyzed (n=3; **P<0.01). UTR, untranslated region; miR, microRNA; NC, normal control