Figure 5.

Resveratrol induces cell death in KYSE150 cells. (A) Resveratrol time-dependently increases cleaved PARP and cleaved caspase-3 expression levels in KYSE150 cells. KYSE150 cells were treated with 80 µM resveratrol at various time points. The whole cell lysates were then extracted and subjected to western blot analysis. The histograms represent the means ± standard deviations from three independent experiments; *P<0.05 and ***P<0.001 for suppression of protein expression by resveratrol vs. DMSO (control) group. (B) Resveratrol time-dependently increases the rate of cell death in KYSE150 cells. Equal numbers of KYSE150 cells in 6-well plates were treated with 80 µM resveratrol for 0, 24, 48 or 72 h. Annexin V-FITC/PI double staining and flow cytometry analysis were used to evaluate resveratrol-induced cell death in KYSE150 cells. Each experiment was conducted in triplicate and independently. The data are presented as the mean ± standard deviation. *P<0.05, increase in resveratrol-induced cell death vs. the control. FITC, fluorescein isothiocyanate; PI, propidium iodide; EGFR, epidermal growth factor receptor; PARP, poly (ADP-ribose) polymerase.