Figure 1.

Process of preparation of PLA-chitosan-IM7 nano-particles. First, the anti-cluster of differentiation 44 antibody IM7 (200 µl) was added to a thiamine pyrophosphate solution (1 mg/ml, pH 7–9) and mixed well. Secondly, the mixture was added to a chitosan solution (pH 4–6), and by molecular reaction, chitosan-IM7 was formed. Thirdly, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide was added to a PLA solution to activate the carboxyl moiety, and then the mixture was added to the chitosan-IM7 nano-particles. The reaction components were mixed thoroughly and were allowed to react for 15 min at room temperature. PLA, polylactic acid; mRNA, messenger RNA.