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. 2016 Oct 20;6(10):189. doi: 10.3390/nano6100189

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of Ag NPs/CNFS or CNFS treatment on oxidative stress in skin. Immunohistochemical (8-hydroxy-2′-deoxyguanosine, 8-OHdG) staining of skin sections was performed to measure the proportion of 8-OHdG-positive sites 1 day after treatment with either Ag NPs/CNFS (A) or CNFS (B). A significant increase in the number of 8-OHdG-positive sites was observed in the Ag NPs/CNFS group compared with the CNFS group. The arrows show the 8-OHdG-positive sites. Each microphotograph is representative of six samples (A,B). The number of 8-OHdG-positive sites in microphotograph of each sample (n = 6) were statistically analyzed (C). Data represent the mean ± SD of 6 determinations, ** Student’s t test, p < 0.01 (C).