Table 1.
LOD, KDeff, and R2 Values Obtained from Fitting the Test Line Intensities As a Function of ZIKV NS1 Concentration in Figure 3 to the Modified Langmuir Binding Isotherm Model for Bare NPs, Preformed Coronas around the NP-Ab, and HS Pretreatment of the Nitrocellulose
| run media |
LOD (nM) |
KDeff (nM) |
R2 | |
|---|---|---|---|---|
| Bare-NP-Ab | HS | 1.6 | 12.7 | 0.96 |
| BSA | --- | --- | --- | |
| PBS | --- | --- | --- | |
| Preformed coronas on NP-Ab | HS | 0.61 | 10.5 | 0.97 |
| BSA | 15.9 | 31.1 | 0.87 | |
| PBS | 1.1 | 11.0 | 0.98 | |
| Paper pretreatment | HS | 0.8 | 17.2 | 0.93 |
| BSA | 2.3 | 10.2 | 0.93 | |
| PBS | 0.2 | 19.0 | 0.98 |