Table 2.

LODs and K_D^eff Values Obtained by Langmuir Isotherm Fits of Test Line Intensities (Figure 5a) and Corresponding R² values^a

MWCO filter	LOD (nM)	KDeff (nM)	R2
Full HS (no filter)	1.6	12.7	0.96
1 MDa	1.1	10.7	0.98
300 kDa	1.0	10.2	0.98
50 kDa	0.88	10.6	0.98
5 kDa	4.7	5.2	0.89

^aFull HS and HS filtered with 1 MDa, 300 kDa, 50 kDa, and 10 kDa MWCO filters were fit to obtain K_D^eff.