Figure 3.

Uptake efficacy of SIDT2 toward different types of nucleic acids. (A) A DNA uptake assay was performed using SIDT2-overexpressing and control lysosomes as described in Materials and Methods. One microgram of circular (plasmid) DNA or linear DNA was used as a substrate. Mean ± SEM (n = 3). ***, P < 0.001; **, P < 0.01; *, P < 0.05. (B) RNA and DNA uptake assays were performed. SIDT2-overexpressing or control lysosomes were incubated with 5 µg of mouse total RNA or linear DNA in the presence of an ATP regeneration system for 5 min at 37°C. Lysosomes were pelleted by centrifugation at 17,700 g for 1 min, and nucleic acids in the supernatant fraction were analyzed by agarose gel electrophoresis. Migration time was 15 min. Mean ± SEM (n = 3). *, P < 0.05.