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. Author manuscript; available in PMC: 2017 Jan 19.
Published in final edited form as: Science. 2016 Sep 9;353(6304):1161–1165. doi: 10.1126/science.aaf5171

Fig. 3. Gene expression in both mouse and human tumors reflects tumor tissue-specific BCAA metabolism.

Fig. 3

(A) Relative Expression of BCAA metabolic pathway genes in normal lung and NSCLC tumors from KP mice. Data are presented as mean ± SEM. N = 6 control and N = 6 NSCLC. (B) Relative expression of BCAA metabolic pathway genes in normal pancreas and PDAC tumors from KP mice. Data are presented as mean ± SEM. N = 7 control and N = 5 PDAC. (C) Immunoblots of proteins involved in BCAA metabolism in representative normal lung and NSCLC tumors (left) and representative normal pancreas and PDAC tumors (right) from KP mice. (D) Quantification of (C). Data are presented as mean ± SEM. N = 6 control and N = 6 NSCLC; N = 4 control and N = 4 PDAC. (E) Comparison of BCAA metabolic pathway gene expression in human NSCLC and PDAC tumors relative to their adjacent paired normal tissues. Overall expression of BCAA metabolism genes is significantly decreased in PDAC (P<0.0001). Two-tailed t test was used for all comparisons between two groups unless otherwise stated. * P<0.05, ** P<0.01, *** P<0.001