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A. and B. Topfluor-LysoPC-tagged host lipids first label the membrane of the MCV, accumulate inside the compartment and are finally found inside the bacteria. Phospholipids of wild type (A) and dgat1&2 DKO (B) were labelled with Topfluor-LysoPC as described in materials and methods. Cells were infected with mCherry-expressing mycobacteria. Images were taken at the indicated time points. Scale bar, 5 μm; Zoom, 2μm.
