Table 2. Differentially abundant phosphoproteins in the Longissimus lumborum muscle of Angus and Nellore bulls.
Match IDa | Protein ID | ABVb | Uniprotaccession number | Mascotscorec | Foldchange | P-valued | % Protein identification probabilitye | Identified peptidesf | % Proteincoverageg | pI observed/expected | MW kDa observed/expected |
---|---|---|---|---|---|---|---|---|---|---|---|
More abundant in Angus | |||||||||||
384 | Troponin T | TNNT3 | Q8MKI4 | 103 | 3.56 | 0.0411 | 100 | 2 | 10 | 6.91/5.93 | 39.8/32.1 |
644 | Phosphoglucomutase-1 | PGM1 | Q08DP0 | 199 | 1.65 | 0.0065 | 100 | 3 | 8 | 6.19/6.36 | 64.9/61.6 |
870 | Phosphoglucomutase-1 | PGM1 | Q08DP0 | 377 | 1.95 | 0.0481 | 100 | 6 | 10 | 6.63/6.36 | 63.6/61.8 |
More abundant in Nellore | |||||||||||
1061 | Myosin light chain 1/3 | MYL1 | A0JNJ5 | 412 | NDh in Angus | 0.0001 | 100 | 5 | 34 | 5.12/4.96 | 24.8/21.0 |
1064 | Myosin light chain 1/3 | MYL1 | A0JNJ5 | 80 | NDh in Angus | 0.0001 | 99 | 1 | 5 | 5.07/4.96 | 25.0/21.0 |
38 | Myosin regulatory light chain 2 | MYLPF | Q0P571 | 212 | 1.45 | 0.0063 | 100 | 3 | 24 | 4.69/4.91 | 18.1/19.0 |
37 | Myosin regulatory light chain 2 | MYLPF | Q0P571 | 113 | 1.26 | 0.0213 | 100 | 1 | 7 | 4.79/4.91 | 17.6/19.0 |
485 | Alpha actin 1 | ACTA1 | A4IFM8 | 465 | 1.82 | 0.0290 | 100 | 6 | 22 | 4.96/5.23 | 43.2/42.3 |
494 | Alpha actin 1 | ACTA1 | A4IFM8 | 213 | 1.29 | 0.0470 | 100 | 2 | 6 | 5.22/5.23 | 43.9/42.3 |
143 | Triosephosphate isomerase | TPI1 | Q5E956 | 550 | 1.31 | 0.0113 | 100 | 6 | 34 | 6.40/6.45 | 26.4/26.9 |
179 | 14-3-3 protein épsilon | YWHAE | P62261 | 219 | 1.25 | 0.0228 | 100 | 3 | 12 | 4.67/4.63 | 26.8/29.3 |
a Match ID correspond to the numbers shown in Fig 2
b Protein abbreviation is in accordance with gene abbreviation in UniProt
c The Mascot baseline significant score is 31
d P-value obtained by ANOVA comparing spots abundance between Angus and Nellore muscle
e Probability for validation by Scaffold software of the proteins identified by Mascot
f Number of peptides identified by Mascot and validated by Scaffold. The sequences of the peptides are found in S2 Table
g Protein coverage calculated by Scaffold (identified amino acids / total amino acids)
h ND spot not detected