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. 2017 Jan 19;12(1):e0170294. doi: 10.1371/journal.pone.0170294

Table 2. Differentially abundant phosphoproteins in the Longissimus lumborum muscle of Angus and Nellore bulls.

Match IDa Protein ID ABVb Uniprotaccession number Mascotscorec Foldchange P-valued % Protein identification probabilitye Identified peptidesf % Proteincoverageg pI observed/expected MW kDa observed/expected
More abundant in Angus
384 Troponin T TNNT3 Q8MKI4 103 3.56 0.0411 100 2 10 6.91/5.93 39.8/32.1
644 Phosphoglucomutase-1 PGM1 Q08DP0 199 1.65 0.0065 100 3 8 6.19/6.36 64.9/61.6
870 Phosphoglucomutase-1 PGM1 Q08DP0 377 1.95 0.0481 100 6 10 6.63/6.36 63.6/61.8
More abundant in Nellore
1061 Myosin light chain 1/3 MYL1 A0JNJ5 412 NDh in Angus 0.0001 100 5 34 5.12/4.96 24.8/21.0
1064 Myosin light chain 1/3 MYL1 A0JNJ5 80 NDh in Angus 0.0001 99 1 5 5.07/4.96 25.0/21.0
38 Myosin regulatory light chain 2 MYLPF Q0P571 212 1.45 0.0063 100 3 24 4.69/4.91 18.1/19.0
37 Myosin regulatory light chain 2 MYLPF Q0P571 113 1.26 0.0213 100 1 7 4.79/4.91 17.6/19.0
485 Alpha actin 1 ACTA1 A4IFM8 465 1.82 0.0290 100 6 22 4.96/5.23 43.2/42.3
494 Alpha actin 1 ACTA1 A4IFM8 213 1.29 0.0470 100 2 6 5.22/5.23 43.9/42.3
143 Triosephosphate isomerase TPI1 Q5E956 550 1.31 0.0113 100 6 34 6.40/6.45 26.4/26.9
179 14-3-3 protein épsilon YWHAE P62261 219 1.25 0.0228 100 3 12 4.67/4.63 26.8/29.3

a Match ID correspond to the numbers shown in Fig 2

b Protein abbreviation is in accordance with gene abbreviation in UniProt

c The Mascot baseline significant score is 31

d P-value obtained by ANOVA comparing spots abundance between Angus and Nellore muscle

e Probability for validation by Scaffold software of the proteins identified by Mascot

f Number of peptides identified by Mascot and validated by Scaffold. The sequences of the peptides are found in S2 Table

g Protein coverage calculated by Scaffold (identified amino acids / total amino acids)

h ND spot not detected