Fig 2. The effect of different concentrations of α-MSH on the electrical resistance of cytokine treated Caco-2 monolayers.
Caco-2 monolayers grown on culture inserts were treated with cytokines (CK; 50 ng/ml TNF-α and 25 ng/ml IL-1β) without or with different concentrations of α-MSH (10−4 M, 10−8 M, 10−12 M, and 10−16 M) for 24 hours. The control group (C) received culture medium. Transepithelial electrical resistance (TEER) values are shown as percentage of untreated control. Means ± S.D., n = 3; ***P < 0.001, *P < 0.05, ###P<0.001. *: CK, CK+MSH compared to C; #: CK+MSH compared to CK.