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. 2017 Jan 6;11(1):e0005272. doi: 10.1371/journal.pntd.0005272

Fig 4. Effects of silencing Piwi5, Piwi6 and Ago3 on BUNV-NL and SBV-NL replication in Aag2 cells.

Fig 4

Silencing of transcripts by transfection of sequence-specific dsRNA (Piwi5, Piwi6, Ago3 or eGFP as negative control) into Aag2 cells, followed by infection at MOI 0.01 at 24 hours p.t.; BUNV-NL (A), SBV-NL (B). Cells were lysed at 48 hours p.i. and luciferase activity determined (A-B). Knockdown of RNAi transcripts was verified by qRT-PCR (C). S7 was used as internal control in qRT-PCR assays. All data were normalized to cells transfected with control dsRNA. Graphs represent three independent experiments performed in triplicate for the luciferase assays and three independent experiments for the qRT-PCR assays. * p≤0.05 student t-test.