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. 2017 Jan 6;13(1):e1006145. doi: 10.1371/journal.ppat.1006145

Fig 7. ZAP synergizes with TRIM25 to block SINV translation.

Fig 7

T-REx-hZAP cells were transfected with TRIM25-targeting or NT pool siRNA, induced for ZAPS expression, and infected with a temperature-sensitive SINV that expresses luciferase and is unable to replicate at 40°C (Toto1101/Luc:ts6; MOI = 10). Following 1 hour of adsorption with virus at 37°C, cells were moved to 40°C, washed, and lysed at 0, 2, 4 and 6 h p.i. for measurement of (A) viral RNA by RT-qPCR, and (B) luciferase activity, which represents translation of the incoming viral genome. The data is representative of 2 independent experiments.