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. 2017 Jan 6;13(1):e1006123. doi: 10.1371/journal.ppat.1006123

Fig 6. NLRP3 interacts with EV71 3D through the NACHT and LRR domains.

Fig 6

(A and B) Human embryonic kidney cells (HEK 293T) were transfected with HA-3D plasmid in combination with plasmids encoding Flag-NLRP3. Lysates were either analyzed directly (30 μg protein; bottom) by immunoblotting using anti-3D and anti-Flag antibody (input) or subjected to IP using control IgG or anti-3D antibody (A) or control IgG and anti-Flag antibody (B) and then analyzed by immunoblotting using anti-3D and anti-Flag antibody. (C) THP-1 cells were infected by control (CT) or 3D-encoding lentivirus. And then THP-1 cells were differentiated into macrophages with TPA. Lysates were either analyzed directly (30 μg protein; bottom) by immunoblotting using anti-NLRP3 and anti-3D antibody (input) or subjected to IP using control IgG or anti-3D (top) antibody and then analyzed by immunoblotting using anti-NLRP3 and anti-3D antibody. (D and E) HEK293T cells were transfected with HA-3D plasmid in combination with plasmids encoding Flag-NLRP3, Flag-PYRIN, Flag-NACHT, or Flag-LRR. Lysates were either analyzed directly (30 μg protein; bottom) by immunoblotting using anti-3D and anti-Flag antibody (input) or subjected to IP using control IgG and anti-3D antibody (D) or control IgG and anti-Flag antibody (E) and then analyzed by immunoblotting using anti-3D and anti-Flag antibody.