Figure 3. miRNA-548c-3p, miRNA-509-3p, and miRNA-23b promote mitosis in ACMs.
A. Representative fluorescence images of ACMs transfected with cel-miR-67(control), miR-548c-3p, miR-509-3p, miR-23b, and all three miRNAs together and stained for TnI (green), DAPI (blue) and pH3 (Red); B. Quantitative analysis of percentage pH3 positive ACMs. Meis1 siRNA = siMeis1. Student t-test performed to determine significance.*p<0.05. Combination= all three miRNAs together at 50nM concentration each.