Skip to main content
. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Nov 28;482(4):1019–1024. doi: 10.1016/j.bbrc.2016.11.150

Fig. 2. Intracellular calcium accumulation upon PTH stimulation is inhibited by preincubating cells with H2O2.

Fig. 2

A) 100 nM NNT-PTH caused rapid increases in Fluo-4 AM fluorescence in HEK-293R cells, corresponding to an elevation of intracellular calcium activity. Pretreatment with 100 μM H2O2 for 15 min decreased calcium signaling triggered by NNT-PTH. B) Detailed changes of Fluo-4 AM after PTH stimulation. Data represent the mean ± SEM of N = 3 independent experiments; n = 17 for H2O2 + NNT-PTH and n = 20 for HBSS + NNT-PTH.