Figure 8. Oligomerization of Mis18 complex promotes robust centromere localization.
(A, B) Representative images showing the fluorescence of CENP-A-SNAP-SiR, mCherry-Mis18α and GST-EGFP-M18BP1 in the fixed HeLa cells treated as described in Figure 2A. Centromeres were visualized with CREST sera. Scale bars represent 10 µm. (C–E) Quantification of the fluorescence intensity of CENP-A-SNAP-SiR (panel C), mCherry-Mis18α (panel D), or EGFP-M18BP1 variants (panel E) on each centromere. The quantification was performed and is presented in the same way described in the legend of Figure 2. P-Values are indicated on the graph (Student’s t test).
Figure 8—figure supplement 1. GST-EGFP-M18BP1141-1132 localizes more specifically to centromeres than EGFP-M18BP1141-1132.
Representative images showing EGFP-M18BP1 fluorescence and the immunofluorescence of CREST sera of indicated HeLa cell lines treated as described in Figure 2A. Scale bars represent 10 µm.