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. 2017 Jan 13;12:577–591. doi: 10.2147/IJN.S111284

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© 2017 Chai et al. This work is published and licensed by Dove Medical Press Limited

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Representative images showing (A) accumulation of PG-AF (A-ii) but not free AF (A-2) in HUVEC after a 1 h incubation. (B) The accumulation of PG-AF in HUVEC was dose dependent. A very low or no fluorescence signal was detected when cells were incubated with increasing concentrations of free AF dye. A-1, A-i depicts blue fluorescence (refers to nucleus stained with DAPI); A-2, A-ii depicts green fluorescence (represents AF compound); A-3, A-iii 3 depicts merged images of A-1 + A-2, and A-i + A-ii, respectively.

Abbreviations: PG-AF, poly-l-glutamic acid-5-(aminoacetamido) fluorescein (fluoresceinyl glycine amide); HUVEC, human umbilical vein endothelial cells; DAPI, 4′,6-diamidino-2-phenylindole⋅2HCl; AF, 5-(aminoacetamido) fluorescein.