Table 1.
Changes in organ/tissue radioactivity (dpm/g tissue) over 24 h in normal and diabetic rats administered with 10 µCi of 17 kDa PG-Tr, 41 kDa PG-Tr, or Free-Tr
| Radiolabel | Time (h) | Radioactivity in various tissues in normal rats (×1,000 dpm/g tissue) | |||||||
|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||
| Intestine | Stomach | Spleen | Kidney | Liver | Lungs | Heart | Muscle | ||
| Free-Tr | 0.25 | 86.0±12.1 | 98.6±13.7 | 105.0±7.9 | 281.9±39.8 | 109.3±17.6 | 78.1±11.7 | 95.3±12.7 | 99.4±15.9 |
| 1 | 156.3±15.2 | 155.6±17.5 | 120.2±21.1 | 189.3±28.7 | 150.3±24.3 | 99.7±14.7 | 125.5±13.3 | 89.7±32.7 | |
| 2 | 77.9±15.8 | 75.4±9.3 | 62.0±6.9 | 75.2±11.0 | 59.5±7.5 | 45.6±7.5 | 54.0±7.7 | 53.7±6.1 | |
| 4 | 107.3±32.3 | 60.8±12.9 | 60.3±10.5 | 71.2±17.6 | 48.0±10.8 | 47.7±12.3 | 48.6±8.1 | 41.3±5.8 | |
| 6 | 83.6±15.5 | 50.0±9.2 | 48.7±5.7 | 54.8±11.8 | 39.0±7.6 | 53.1±14.9 | 44.5±7.9 | 37.5±2.6 | |
| 8 | 94.3±19.1 | 62.4±9.3 | 51.4±5.7 | 54.7±9.3 | 43.5±5.8 | 44.5±6.3 | 42.7±6.1 | 43.4±6.3 | |
| 12 | 157.8±18.6 | 89.1±12.4 | 77.1±12.7 | 84.9±10.9 | 70.9±12.2 | 68.9±14.5 | 61.1±8.0 | 60.0±14.6 | |
| 24 | 101.5±28.5 | 51.8±11.7 | 37.4±5.7 | 44.8±8.6 | 24.5±0.6 | 41.6±10.2 | 36.1±5.4 | 37.5±1.4 | |
| 17 kDa PG-Tr | 0.25 | 97.5±34.7 | 81.4±26.7 | 87.6±26.4 | 2,312.3±127.0 | 236.4±19.5 | 173.4±61.7 | 190.3±102.8 | 70.9±25.5 |
| 1 | 53.7±37.6 | 29.9±18.2 | 88.6±29.9 | 1,168.7±144.6 | 260.3±42.3 | 106.2±9.8 | 34.1±22.4 | 26.0±18.3 | |
| 2 | 154.5±53.8 | 74.9±25.5 | 113.2±27.5 | 941.2±60.6 | 226.5±19.4 | 83.3±8.2 | 69.7±23.1 | 64.0±24.1 | |
| 4 | 165.3±58.5 | 80.3±26.6 | 120.5±27.7 | 835.8±76.8 | 247.6±45.2 | 86.7±7.2 | 53.2±16.9 | 67.5±24.2 | |
| 6 | 174.8±66.4 | 75.8±24.8 | 78.7±16.8 | 627.8±33.7 | 204.8±37.6 | 77.7±10.1 | 52.8±16.5 | 60.1±20.5 | |
| 8 | 149.9±52.1 | 60.6±20.5 | 100.2±17.3 | 503.0±15.8 | 235.3±60.9 | 69.0±6.5 | 55.4±14.4 | 55.1±18.9 | |
| 12 | 201.7±72.9 | 80.8±27.7 | 87.4±12.0 | 366.4±10.7 | 168.6±26.7 | 64.4±6.4 | 51.5±17.5 | 61.9±21.1 | |
| 24 | 171.5±59.4 | 65.7±21.4 | 73.2±12.3 | 323.6±23.7 | 141.1±32.5 | 62.1±6.3 | 43.0±13.5 | 42.9±14.2 | |
| 41 kDa PG-Tr | 0.25 | 134.6±23.0 | 66.5±10.8 | 229.2±43.7 | 2,540.4±209.1 | 733.7±105.0 | 106.3±20.6 | 70.8±7.6 | 42.1±4.3 |
| 1 | 233.0±76.5 | 44.2±10.1 | 246.4±57.7 | 2,511.7±159.2 | 1,115.6±167.8 | 125.5±30.4 | 38.4±9.5 | 25.4±6.0 | |
| 2 | 227.9±64.4 | 68.9±5.0 | 432.9±109.4 | 1,983.5±260.1 | 1,020.1±198.7 | 113.7±24.7 | 53.6±11.4 | 39.0±4.9 | |
| 4 | 121.8±30.9 | 45.5±9.9 | 255.7±102.0 | 1,475.6±151.0 | 491.0±54.0 | 148.8±30.8 | 45.8±10.1 | 41.9±7.4 | |
| 6 | 186.0±49.5 | 76.7±23.0 | 488.1±194.6 | 1,204.6±131.2 | 305.2±64.3 | 124.4±17.8 | 52.9±18.6 | 43.4±13.6 | |
| 8 | 93.1±23.0 | 56.3±13.3 | 226.9±109.6 | 957.9±117.2 | 238.9±60.2 | 79.1±19.1 | 41.4±11.8 | 34.5±9.3 | |
| 12 | 110.1±39.5 | 67.6±19.2 | 249.0±107.3 | 1,033.0±161.0 | 298.6±32.2 | 68.4±27.0 | 34.8±11.8 | 35.3±12.5 | |
| 24 | 128.0±40.8 | 70.7±14.5 | 207.8±87.2 | 666.8±52.5 | 264.4±77.3 | 113.9±36.1 | 47.2±11.9 | 50.4±10.9 | |
Note: Each data point is expressed as mean ± SEM (n=5).
Abbreviations: dpm, disintegration per minute per mL; PG, poly-l-glutamic acid; PG-Tr, radiolabeled poly-l-glutamic acid; Free-Tr, free unbound radiolabels; SEM, standard error of mean.