Table 2.
Changes in organ/tissue radioactivity (dpm/g tissue) over 24 h in diabetic rats administered with 10 µCi of 17 kDa PG-Tr, 41 kDa PG-Tr, or Free-Tr
| Radiolabel | Time (h) | Radioactivity in various tissues in diabetic rats (×1,000 dpm/g tissue) | |||||||
|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||
| Intestine | Stomach | Spleen | Kidney | Liver | Lungs | Heart | Muscle | ||
| Free-Tr | 0.25 | 139.8±19.6 | 92.4±12.8 | 104.2±22.4 | 174.4±19.9 | 117.1±20.3 | 84.1±12.4 | 95.1±14.7 | 93.6±14.9 |
| 1 | 197.3±23.2 | 155.4±7.5 | 94.4±6.6 | 187.5±5.5 | 155.8±5.9 | 105.5±2.7 | 111.0±7.2 | 120.6±7.4 | |
| 2 | 180.8±9.6 | 137.5±18.3 | 92.1±12.7 | 163.1±12.2 | 136.3±14.6 | 90.5±9.8 | 104.6±10.6 | 97.8±11.1 | |
| 4 | 155.0±8.3 | 103.8±13.5 | 63.1±14.1 | 99.5±6.9 | 92.8±7.2 | 77.3±6.4 | 82.1±5.9 | 84.8±6.3 | |
| 6 | 159.5±7.7 | 118.1±5.2 | 73.1±10.4 | 104.3±4.7 | 100.5±4.7 | 80.7±8.9 | 74.2±5.3 | 89.9±6.0 | |
| 8 | 211.4±33.0 | 114.7±9.9 | 73.3±5.2 | 111.0±9.9 | 107.3±12.4 | 81.5±3.9 | 89.0±7.2 | 93.0±7.8 | |
| 12 | 144.0±19.9 | 74.4±11.2 | 52.4±2.8 | 70.2±8.4 | 63.1±6.7 | 60.8±3.7 | 57.9±6.1 | 67.1±7.6 | |
| 24 | 181.4±5.9 | 84.5±4.7 | 44.5±0.9 | 74.2±3.8 | 69.1±4.0 | 50.2±3.0 | 55.1±2.1 | 60.9±3.8 | |
| 17 kDa PG-Tr | 0.25 | 18.4±5.2 | 21.5±7.1 | 42.9±11.5 | 169.2±66.7 | 284.7±141.4 | 219.0±53.7 | 30.5±15.2 | 39.0±13.3 |
| 1 | 25.3±13.6 | 25.7±13.5 | 61.2±26.3 | 138.5±37.4 | 350.3±139.1 | 164.4±13.5 | 16.5±5.6 | 33.0±13.3 | |
| 2 | 116.4±43.5 | 66.3±17.2 | 86.4±13.3 | 554.8±21.7 | 260.6±56.8 | 103.8±14.8 | 60.4±19.7 | 65.6±22.2 | |
| 4 | 152.5±38.1 | 41.1±13.2 | 65.9±11.5 | 447.5±32.1 | 155.6±33.8 | 67.9±2.8 | 48.3±9.8 | 31.0±5.7 | |
| 6 | 104.9±32.4 | 47.5±12.5 | 51.1±6.0 | 383.0±20.7 | 149.1±42.5 | 52.9±7.9 | 46.8±12.8 | 22.2±3.6 | |
| 8 | 97.6±32.1 | 39.6±11.3 | 46.7±7.5 | 338.4±13.7 | 194.3±50.6 | 70.4±6.0 | 39.1±11.6 | 20.8±5.5 | |
| 12 | 54.2±13.1 | 20.5±3.9 | 38.9±6.3 | 153.4±6.7 | 92.9±21.1 | 94.0±36.8 | 20.2±4.0 | 32.7±10.4 | |
| 24 | 104.7±32.1 | 36.2±11.0 | 53.7±5.1 | 291.8±48.2 | 183.6±60.8 | 57.9±7.4 | 37.9±10.0 | 31.9±9.4 | |
| 41 kDa PG-Tr | 0.25 | 91.4±25.0 | 82.9±20.1 | 75.2±18.1 | 1,459.8±168.6 | 174.4±18.7 | 67.0±18.7 | 64.5±15.8 | 55.2±14.2 |
| 1 | 285.8±96.4 | 85.3±26.0 | 62.3±12.0 | 1,148.8±166.3 | 176.8±50.5 | 59.4±10.0 | 58.7±6.6 | 66.1±19.7 | |
| 2 | 337.7±134.9 | 68.4±7.9 | 76.2±9.4 | 964.9±75.9 | 145.8±17.2 | 58.9±6.9 | 49.7±5.3 | 58.3±7.3 | |
| 4 | 136.1±17.9 | 65.3±5.4 | 58.4±2.5 | 824.7±40.5 | 131.5±18.9 | 42.6±3.1 | 45.6±2.7 | 40.6±1.7 | |
| 6 | 128.4±21.6 | 49.2±4.5 | 54.2±5.9 | 1,031.5±104.9 | 129.2±17.9 | 46.7±3.9 | 44.1±1.7 | 47.6±3.8 | |
| 8 | 114.6±13.8 | 42.3±3.0 | 53.7±5.2 | 741.4±101.8 | 106.7±14.3 | 41.7±3.7 | 38.6±1.8 | 48.5±3.5 | |
| 12 | 134.6±13.8 | 54.2±4.4 | 96.7±27.3 | 548.6±88.4 | 141.4±30.8 | 64.0±4.9 | 47.2±3.4 | 51.6±7.0 | |
| 24 | 126.3±18.5 | 45.1±6.5 | 229.7±99.7 | 691.6±91.1 | 116.2±15.0 | 58.0±11.1 | 45.5±7.0 | 60.4±4.9 | |
Note: Each data point is expressed as mean ± SEM (n=5).
Abbreviations: dpm, disintegration per minute per mL; PG, poly-l-glutamic acid; PG-Tr, radiolabeled poly-l-glutamic acid; Free-Tr, free unbound radiolabels; SEM, standard error of mean.