Figure 8.

The Imd pathway regulates the local expression of Diptericin and Drosomycin in larvae after infection by Gram-negative bacteria. (A–C). The expression of the Diptericin-lacZ reporter gene in the midgut after natural infection by E. carotovora 15 is blocked in dTAK1¹ and Dredd^B118 mutants. Wild-type (A), dTAK1¹ (B), and Dredd^B118 (C) mutant larvae carrying the Diptericin-lacZ reporter gene were collected 24 h after natural infection by E. carotovora 15 at 29°C and stained for lacZ activity as described (Tzou et al. 2000). (D-F). The expression of the Drosomycin-GFP reporter gene in trachea after natural infection by E. carotovora 15 is blocked in dTAK1¹ and Dredd^B118 mutants. Wild-type (D), dTAK1 (E), and Dredd^B118 (F) larvae carrying the Drosomycin-GFP reporter gene were collected 5 d after natural infection by E. carotovora 15 at 18°C and directly observed under an epifluoresence microscope.