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. 2003 Apr;15(4):899–913. doi: 10.1105/tpc.009670

Figure 6.

Figure 6.

Analysis of ADL1A, ADL1E, and ADL1A-mGFP5 Expression in Wild-Type and Mutant Plants.

(A) Immunoblot analysis of total protein prepared from rosette leaves of wild-type (lane 1), heterozygous adl1A-2 (lane 2), homozygous adl1A-2 (lane 3), and homozygous adl1E-1 (lane 4) plants. The relative mobilities of the ADL1 proteins in lanes 3 and 4 were confirmed by analysis of a protein mixture containing both homozygous adl1A-2 and homozygous adl1E-1 protein extracts (lane 5). The immunoblots were probed using α-GTPase, α-ADL1A, or α-AtCDC48 antibody. Abbreviations are as in Figure 5.

(B) Immunoblot analysis of transgenic lines expressing ADL1A-mGFP5. Total protein was prepared from rosette leaves of transgenic heterozygous adl1A-2 (lane 1), homozygous adl1A-2 (lane 2), and wild-type (lane 4) plants and untransformed heterozygous adl1A-2 plants (lane 3). The immunoblots were probed using α-GTPase or α-GFP antibody.