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. Author manuscript; available in PMC: 2017 Feb 18.
Published in final edited form as: Nature. 2016 Aug 18;536(7616):292–297. doi: 10.1038/nature19097

Extended Data Figure 4. Co-expression of CRY-GAL80 or TNT blocks the sleep-promoting effect of DN1 activation.

Extended Data Figure 4

a, R18H11-GAL4/UAS-CsChrimson (left panel) and R18H11-GAL4, CRY-GAL80/UAS-CsChrimson (right panel) brains were dissected and stained with anti-GFP (green). Scale bar=100 μm. b, Comparison of total sleep in the baseline day (blue) to total sleep during a 24 hour LED stimulation day (red) for each genotype. n=32 for R18H11-GAL4/UAS-CsChrimson group and n=24 for the other groups. Error bars represent SEM. ‘**’ indicates p<0.001 by post-hoc Bonferonni multiple comparisons. Two-way ANOVA detected a significant LED stimulation effect (p=0.00227674), a genotype effect (p<0.0001) and interaction (p<0.0001).