Figure 5.
YBX1 regulated RCC cell migration and invasion was mediated by AR. SW839 cells were transfected with pLK0.1-scr, pLK0.1-YBX1, pWPI-AR, or pLK0.1-YBX1 together with pWPI-AR plasmids. (A) YBX1 and AR expression in SW839 cell lines were tested by Western blot. (B) 1 × 105 SW839 cells were plated onto the upper matrigel coated transwell chambers with 8 μm pore polycarbonate membrane for cell invasion assay. (C) 5 × 104 SW839 cells were plated onto the upper chamber with 8 μm pore polycarbonate membrane for cell migration assay. (**P < 0.01).