Figure 6.

Lamp2a levels at the lysosomal membrane increase during mild oxidative stress. (A and B) Immunoblot for lamp2a and lamp1 of homogenates (50 μg of protein), lysosomal membranes (L. Memb) and lysosomal matrices (L. Matrix) (10 μg of protein) isolated from cultured mice fibroblasts, exposed or not to 100 μM H₂O₂ (A) or from liver of rats treated or not with paraquat (PQ). (C) Imunoblot for lamp2a, lamp2c, and lamp1 of lysosomal membranes isolated from livers of fed rats, 48-h starved (Strv) rats, or fed rats treated with paraquat (PQ). Right, densitometric quantification of six to eight immunoblots as the ones shown here. Values are expressed as fold of the values in fed untreated animals and are the mean + SE of six different experiments. Levels in lysosomes from fed animals were given an arbitrary value of 1. ^*, differences compared with fed animals (^*p < 0.05, ^**p < 0.01, ^***p < 0.001).