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a Genomic locus of targeted exon 2. b Location of guides flanking exon 2 of the TMPRSS2 gene. +1 represents the A in the start codon ATG. Guides 1 + 5, 2 + 4 and 3 + 6 were mixed and coinjected. A designed deletion would result in the removal of exon 2 and the start codon
