Figure 2.

Morphologic studies of small intestine in 4 mouse lines. (A) H&E staining in small intestine. Scale bar: 200 μm. The enlargement of Paneth cells with characteristic eosinophilic staining was observed in the crypt of Int-G_q/G₁₁ DKO mice. Slightly enlarged Paneth cells also were observed in G₁₁ KO mice. In contrast, the alteration was not observed in Int-G_q KO mice. (B) Transmission electron micrographs in the crypt. Scale bars: 10 μm. Paneth cells in G_q/G₁₁-deficient crypts showed a bipartite structure in their secretory granules: a smaller electron-dense core and a peripheral halo. An unusually large granulomucous cell also was observed (arrow). (C) Unhealthy-looking Paneth cells in G_q/G₁₁-deficient crypts. Scale bars: 10 μm. Electron-dense materials might be degraded in a cell.