Figure 5.

Reduction in Wnt/β-catenin signaling and a subtle alteration in Notch signaling in Gα_q/11-deficient IECs. Immunohistochemical studies were performed for (A) β-catenin, (B) Tcf1, (C) Sox9, (D) Dll1, and (E) Hes1. Immunoreactivities for nuclear β-catenin, Tcf1, and Sox9 were reduced in the crypt base of G₁₁ KO and greatly in Int-G_q/G₁₁ DKO mice compared with control and Int-G_q KO mice. Scale bars: 100 μm. Western blot was performed for the (F) Tcf1 and (G) NICD1. Lower panel: ImageJ densitometry analysis. (H) The relative mRNA expression in the Notch signaling molecules was evaluated by a quantitative real-time PCR. The data represent the means ± SD of 3 mice per genotype. Two independent experiments were performed with similar results (n = 3). *P < .05, **P < .01.