Supplementary Table 1.

The Primers Used in the Present study

Gene name		Accession no		Primer sequence	Size	Annealing temperature
Genotyping
Gna11+			F	GCCCCTTGTACAGATGGCAG	820 bp	60°C
			R	AGCATGCTGTAAGACCGTAG
Gna11-			F	CAGGGGTAGGTGATGATTGTGC	450 bp	60°C
			R	GACTAGTGAGACGTGCTACTTCC
Gnaq+			F	AGCTTAGTCTGGTGACAGAAGC	600 bp	60°C
Gnaqflox			R	GCATGCGTGTCCTTTATGTGAG	700 bp
Cre recombinase			F	CAATTTACTGACCGTACAC	1026 bp	60°C
			R	TAATGCCATCTTCCAGCAG
Reverse-transcription PCR
Gnaq		NM_008139	F	TGGACCGTGTAGCCGACCCT	270 bp	60°C
			R	GTGCTTTGCTCTCCTCCATGCGG
Gna11		NM_010301	F	ACGAGGTGAAGGAGTCGAAGC	573 bp	60°C
			R	CCATCCTGAAGATGATGTTCTCC
Actb		NM_007393	F	TGAGAGGGAAATCGTGCGTG	460 bp	60°C
			R	GATCCACATCTGCTGGAAGGTG
Real-time PCR
1	PKCα	NM_011101	F	GCTTCCAGTGCCAAGTTTGC	76 bp	60°C
			R	GCACCCGGACAAGAGAACGTAA
2	PKCδ	NM_011103	F	TATCAACTGGTCCCTCCTGG	105 bp	60°C
			R	CATTCAGGAACTCTGGGTCA
3	Lyz	NM_013590	F	GGTCTACAATCGTTGTGAGTTGG	387 bp	65°C
			R	CTCCGCAGTTCCGAATATACT
4	Defa1	NM_010031	F	TCAAGAGGCTGCAAAGGAAGAGAAC	94 bp	60°C
			R	TGGTCTCCATGTTCAGCGACAGC
5	EphB3	NM_010143	F	CCATAGCCTATCGGAAGTTTACGT	87 bp	60°C
			R	TCGCTCTCCGTAGCTCATGAC
6	EphB2	NM_001290753	F	CTCTACTGTAACGGGGACGG	119 bp	60°C
			R	TTGAAGGTTCCTGATGGACA
7	Fzd5	NM_001042659	F	GTGCTTCATCTCCACGTCCA	103 bp	60°C
			R	CAGGTAGCACGCAGACAAGA
8	Fzd7	NM_008057	F	ACCCTACTGCTCCCTACCTG	84 bp	60°C
			R	AGAAGGGGAAAGACAAGCGG
9	Wnt5a	NM_009524	F	ACAGGCATCAAGGAATGCCA	104 bp	60°C	+DMSO
			R	CGGCTGCCTATTTGCATCAC
10	Tcf4	NM_013685	F	AGCCCGTCCAGGAACTATG	101 bp	60°C
			R	TGGAATTGACAAAAGGTGGA
11	Ascl2	NM_008554	F	TCCAGTTGGTTAGGGGGCTA	107 bp	60°C
			R	GCATAGGCCCAGGTTTCTTG
12	Axin2	NM_015732	F	TGAGATCCACGGAAACAGC	104 bp	60°C
			R	GTGGCTGGTGCAAAGACAT
13	Dll1	NM_007865	F	GGAGAAGATGTGCGACCCT	103 bp	60°C
			R	CTCCCCTGGTTTGTCACAGT
14	Dll4	NM_019454	F	ATGGGGAGGTCTGTTTTGTG	101 bp	60°C
			R	TATAACCCTTTGGCCCACTG
15	Notch1	NM_008714	F	CAAGAGGCTTGAGATGCTCC	132 bp	65°C
			R	AAGGATTGGAGTCCTGGCAT
16	Hes1	NM_008235	F	CTGGTGCTGATAACAGCGGA	80 bp	60°C
			R	AGGGCTACTTAGTGATCGGT
17	Hes5	NM_010419	F	CAAGGAGAAAAACCGACTGCG	188 bp	60°C	+DMSO
			R	CGAAGGCTTTGCTGTGTTTCA
18	Atoh1	NM_007500	F	GTGCGATCTCCGAGTGAGAG	108 bp	60°C
			R	GGGATAAGCCCCGAACAACA
19	Lgr5	NM_010195	F	CAACCTCAGCGTCTTCACCT	96 bp	60°C	+DMSO
			R	TCTTCTAGGAAGCAGAGGCG
20	Bmi1	NM_007552	F	CTTTCATTGTCTTTTCCGCC	90 bp	60°C
			R	TGGTTGTTCGATGCATTTCT
21	Spdef	NM_013891	F	CACGTTGGATGAGCACTCGCTA	142 bp	60°C
			R	AGCCACTTCTGCACGTTACCAG
22	Gfi1	NM_010278	F	GGCAAAAGATTCCACCAGAA	110 bp	60°C
			R	TTGGAGCTCTGACTGAAGGC
23	Cdx1	NM_009880	F	GACGCCCTACGAATGGATGC	80 bp	60°C
			R	ACTTGTCCTTGGTTCGGGTC
24	Cdx2	NM_007673	F	CCTACCCACGAACAGCATCTACT	69 bp	60°C
			R	CCTGAGGTCCATAATTCCACTCA
25	Stk11 (Lkb1)	NM_001301853	F	CTACTCCGAGGGATGTTGGA	115 bp	60°C
			R	GATAGGTACGAGCGCCTCAG
26	Ihh	NM_010544	F	GACTCATTGCCTCCCAGAACTG	150 bp	60°C
			R	CCAGGTAGTAGGGTCACATTGC
27	Fgfr3	NM_001163215	F	TCGTGGCTGGAGCTACTTC	95 bp	60°C
			R	CTCCTGCTGGCTAGGTTCAG
28	Actb	NM_007393	F	CTTCCTCCCTGGAGAAGAGC	101 bp	60°C
			R	AAGGAAGGCTGGAAAAGAGC

DMSO, dimethyl sulfoxide; F, forward; Lgr5, leucine-rich repeat-containing G-protein-coupled receptor 5; R, reverse; Spdef, SAM pointed domain-containing Ets transcription factor.