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. 2016 Oct 21;3(1):51–71. doi: 10.1016/j.jcmgh.2016.10.001

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

In situ validation of ATOH1 targetome. Fresh-frozen ileal tissues were generated from Atoh1 deletion (Fabp1^Cre; Atoh1^lox/lox; knockout [KO]) or littermate control (Fabp1^Cre; Atoh1^+/+; wild type [WT]) mice. The mRNA expression of ATOH1 target genes, including (A) Nfia, (B) Hepacam2, (C) Krt18, (D) Txndc11, (E) Atxn1, and (F) Sh3bgrl3, were shown by in situ hybridizations. Light periodic acid–Schiff staining was performed after in situ hybridization to provide contrast for imaging. Scale bars: 100 μm.