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. 2017 Jan 20;8:35. doi: 10.3389/fmicb.2017.00035

FIGURE 2.

FIGURE 2

Role of BcSUN1 in cell wall integrity. (A) Growth rate of the indicated strains in various media: 6% tomato leaf extract (Leaf), 25% tomato fruit extract (Fruit), minimal medium (GB5), rich medium (YGG), YGG + 0.005% congo red (CR), YGG + 0.4% boric acid (BA), YGG + 0.05% Calcofluor white (CW), YGG + 1 M Sorbitol (SOR), or YGG + 0.02% SDS (SDS). Results are shown as mean ± SD for 9 biological replicates. Asterisks indicate a statistically significant difference (by t-test, p = 0.05) between the wild type and the mutant in a given medium, and the percentage of reduction in growth rate for the mutant, as compared to the wild type, is indicated above bars. (B) Production of protoplasts (mean ± SD, n = 3) from the indicated strains when treated with a cocktail of cell wall degrading enzymes. (C) Percentage of germinated conidia with two or more germ tubes at 16 h after inoculation in YGG medium (n ≥ 100). (D) Percentage of hyphae with two or more ramifications at 16 h after inoculation in the same medium (n ≥ 100).