FIGURE 7.

Surface interaction between knockout Bcsun1 mutants and plant host. (A) Percentage of plugs released from tobacco leaves by washing with water (for the times indicated at the bottom) at 24 h after inoculation (n ≥ 15). (B) Adhesion strength of mycelium plugs to tobacco leaves after 24 h of incubation (mean ± SD; n = 15). (C) Number of infection cushions produced by the indicated strains from agar plugs on glass slides. The data correspond to the average number of cushion determined from 10 microscopic fields randomly chosen. (D) Production of H₂O₂ (as detected with DAB) in infected tobacco leaves, 36 h after inoculation with conidia of the indicated strains. Upper panel shows an example of the results, and lower panel shows a semi-quantification of H₂O₂ produced, expressed as the percentage of brown pixels in a circumference of constant area (calculated with the software Fiji, mean ± SD; n = 5). (E) Secretion of H₂O₂ by the indicated strains grown for 4 days in YGG medium, detected with DAB.