Table 1.
Transcript levels of genes of shikimate/phenylpropanoid/benzoate pathway measured in 24 and 48 h after infection with F. oxysporum presented as fold of the non-treated control; “–” symbol stands for no change in the transcript expression or change in the range between 0.5 and 2-fold of the control.
| Pathway | Gene | Acc. number | X-fold of the control | |
|---|---|---|---|---|
| 24 h | 48 h | |||
| CORE ROUTE | Shikimate dehydrogenase (SD) | AFSQ01026985 | − | 0.08 |
| AFSQ01002620 | − | 0.36 | ||
| Chorismate synthase (CS) | AFSQ01008633 | 2.23 | − | |
| Chorismate mutase (CM) | AFSQ01018652 | 2.04 | − | |
| Phenylalanine ammonia lyase (PAL) | AFSQ01008390/91 | 8.21 | − | |
| AFSQ01000737 | 5.45 | − | ||
| Trans-cinnamate 4-monooxygenase (C4H) | AFSQ01009050 | 2.23 | − | |
| AFSQ01011791 | 4.62 | − | ||
| BENZOATE ROUTE | 3-ketoacyl-CoA thiolase 2 (β-ketothiolase) | 3.19 | 3.1 | |
| Benzoate/salicylate carboxymethyltransferase (BSMT) | AFSQ01004321 | 7.97 | 18.83 | |
| AFSQ01007680 | 3.43 | 9.94 | ||
| AFSQ01004321 | 9.1 | − | ||
| AFSQ01026389 | 6.27 | − | ||
| AFSQ01007680 | 2.83 | − | ||
| Benzyl alcohol O-benzoyltransferase (BBT) | AFSQ01022016 | 5.33 | − | |
| LIGNIN ROUTE | 4-coumarate-CoA ligase (4CL) | AFSQ01020997 | 3.8 | − |
| AFSQ01023785 | 2.8 | − | ||
| AFSQ01020997 | 4.41 | − | ||
| AFSQ01013215 | 0.04 | 0.09 | ||
| AFSQ01014596 | − | 0.45 | ||
| AFSQ01012878 | − | 2.03 | ||
| Hydroxycinnamoyl-CoA:quinate/shikimate Hydroxycinnamoyl transferase (HCT) | AFSQ01015754 | 2.97 | 3.27 | |
| AFSQ01006651 | 2.17 | − | ||
| Caffeoyl shikimate esterase (CSE) | AFSQ01017841 | 2.77 | 0.12 | |
| AFSQ01011162 | 0.29 | 0.41 | ||
| Caffeic acid 3-O-methyltransferase (COMT) | AFSQ01002471 | 2.99 | − | |
| AFSQ01027305 | − | 0.08 | ||
| AFSQ01024646 | − | 0.18 | ||
| Caffeoyl-CoA O-methyltransferase (CCoAOMT) | AFSQ01027261 | 3.9 | − | |
| Cinnamoyl-CoA reductase (CCR) | AFSQ01007861 | 2.1 | − | |
| AFSQ01010549 | 2.72 | − | ||
| AFSQ01011029 | 10.2 | − | ||
| Cinnamyl alcohol dehydrogenase (CAD) | AFSQ01005963 | 3.46 | 0.44 | |
| FLAVONOID ROUTE | chalcone synthase (CHS) | AFSQ01005163 | 4.7 | − |
| AFSQ01007984 | 4.25 | 74.94 | ||
| AFSQ01007984 | − | 75.33 | ||
| AFSQ01007986 | − | 0.01 | ||
| Flavanone 3-hydroxylase (F3H) | AFSQ01016891 | 6.02 | 2.57 | |
| AFSQ01009941 | 0.02 | − | ||
| AFSQ01025118 | − | 2.49 | ||
| AFSQ01022073 | − | 10.29 | ||
| Isoflavone 2′-hydroxylase (F2′H) | AFSQ01000408 | 20.57 | 3.7 | |
| AFSQ01007109 | 0.23 | 0.4 | ||
| Flavonoid 3′-hydroxylase/monooxygenase (F3′H) | AFSQ01028139 | 3.99 | − | |
| AFSQ01019455 | − | 36.51 | ||
| AFSQ01008368/69 | − | 0.34 | ||
| Flavonoid 3′,5′-hydroxylase (F3',5′H) | AFSQ01012300 | 2.34 | − | |
| leucoanthocyanidin dioxygenase (LDOX) | AFSQ01021358 | 76.64 | 13.54 | |
| AFSQ01012389 | − | 5.11 | ||
| Anthocyanidin reductase (ANR) | AFSQ01023196 | 0.36 | 0.27 | |
| Udp-glucosyltransferase (UGT) | AGD95009 | 38.39 | − | |
Presented results are statistically significant at p < 0.001 and based on three biological replicates. Accession numbers of the sequences of isoforms are provided.