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. 2017 Jan 20;8:32. doi: 10.3389/fimmu.2017.00032

Figure 3.

Figure 3

Loss of CD44 surface expression marks T-cell commitment in vitro. (A) Sorting strategy for isolating T-cell differentiation stages A–F from duplicate (n = 2) 12- and 18-day cocultures started with pooled CD34+ hematopoietic stem cells, based on surface markers CD45, CD34, CD7, CD5, CD1a, and CD44. (B) Hierarchical clustering (Pearson average) of sorted populations A–F using 47 selected T-cell development genes. Expression values were obtained by Taqman array microfluidic cards, followed by ANOVA to remove day and pool effects.