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pH dependence of transglycosylation and hydrolysis. Specific initial reaction rate (r) for hydrolysis (diamonds) and transglycosylation (squares) for TnBgl1A in 85 % hexanol 15 % 0.1 M buffer systems with p-nitrophenyl-β-d-glucopyranoside as glycosyl donor. Alongside the wild-type enzyme (a), N220C (b), N220L (c), N220D (d), N220R (e), N220F (f), and N220Y (g) variants are presented. Error bars represent 1σ
