Table 3.
Tryptophan operon genes affected in the B. subtilis Trp mutant strains (M) compared to the wild type strains (WT)
| Gene | Function | Gene expression ratioa | |
|---|---|---|---|
| M1/WT1 | M2/WT2 | ||
| trpA | Tryptophan synthase alpha chain | 2.8 | 12 |
| trpB | Tryptophan synthase beta chain | 4.1 | 15 |
| trpC | Indole-3-glycerol phosphate synthase | 4 | 20 |
| trpD | Anthranilate phosphoribosyltransferase | 4.2 | 26 |
| trpE | Anthranilate synthase component 1 | 5.2 | 29 |
| trpF | N-(5’-phosphoribosyl)anthranilate isomerase | 9.2 | 20 |
| trpP | Tryptophan transport protein | 1.3 | −1.1 |
| trpS | Tryptophanyl-tRNA synthase | −2.9 | −1.3 |
| pyrH | UMP kinase | −1.2 | 1.3 |
| mtrB | Regulating the trp operon | −1.9 | −1.9 |
| ykvU | Sporulation protein | −3.5 | −21 |
aPrimers were added at 300 nM. The primer efficiencies were in the range of 85–102%. The gyrA and gyrB genes were selected as multiple reference genes. PCR conditions: 20 s at 95 °C, followed by 40 cycles of 3 s of denaturation at 95 °C and 1 min of priming/elongation at 60 °C. The standard deviation of the RT-PCR results was up to 20%