TABLE 2.
Summary of the variety of epitope tags used in this study and the phenotypes of endogenous tagging
| N-tag TERT | Size of insertion (bp) | Expression | Telomerase activity | Telomere length | Repeat addition processivity |
|---|---|---|---|---|---|
| Halo-GS10 | 966 | Lower than wta | Lower than wta | Shorter than wta | |
| Flag-GS20 | 210 | Higher than wt | wt level | Shorter than wt | |
| YbbR | 153 | Lower than wt | Lower than wt | Shorter than wt | |
| Flag-GS10 | 144 | Higher than wt | ∼1.3-fold higher than wt | wt level | |
| Flag | 78 | wt level | wt level | Shorter than wt | Low |
| HA | 39 | Higher than wt | Lower than wt | Shorter than wt | Low |
Data not shown. wt, wild type.