Skip to main content
. 2017 Jan 19;37(3):e00279-16. doi: 10.1128/MCB.00279-16

FIG 3.

FIG 3

Asc1 protein abundance in phosphosite mutant strains. (A to C) Immunodetection of Asc1p within cell extracts derived from ASC1 wild-type, asc1DE, and phosphosite mutant strains and the asc1− strain (negative control). Ponceau red staining of proteins served as a loading control, and a part of the lower half of the stained Western blot membrane is depicted. The graphs provide mean relative intensities for three replicates, with standard deviations indicated by error bars. Fold changes were calculated relative to wild-type signal intensities. All nine values for the asc1DE strain were taken together. Panel C further shows Northern blots for detection of ASC1 mRNA and SNR24 snoRNA U24 levels in T143 phosphosite mutant strains. rRNA served as a loading control.